MicroRNA Expression Profiles of Epicardial Adipose Tissue-Derived Exosomes in Patients with Coronary Atherosclerosis

Background and Aims: Epicardial adipose tissue, exosomes, miRNAs have important activities in atherosclerosis. The purpose of this study was to establish miRNA expression profiles epicardial tissue-derived exosomes patients with coronary Methods: Biopsies tissue were obtained from without artery disease (CAD, n = 12 NCAD, 12) during elective open-heart surgeries. Tissue incubated DMEM-F12 for 24 hours. Exosomes isolated, then nanoparticle tracking analysis, transmission electron microscopy, immunoblotting performed confirm the existence exosomes. Total RNA subjected high-throughput sequencing identify differentially expressed miRNAs. MicroRNA target gene prediction performed, genes analyzed by Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG), mirPath function. Reverse transcription quantitative PCR Results: Fifty-three unique identified (adjusted p < 0.05, fold change >2), among which 32 upregulated 21 downregulated patients. validated results seven including miR-141-3p, miR-183-5p, miR-200a-5p, miR-205-5p, miR-429, miR-382-5p miR-485-3p, last two downregulated. GO KEGG analysis indicated that these enriched cell survival, apoptosis, proliferation, differentiation. Conclusions: Coronary showed differential exosomal compared disease. provide clues further studies mechanisms